Characterization of Xanthomonas phaseoli Bacteriophages

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Characterization of Xanthomonas phaseoli Bacteriophages.

Ten bacteriophages for Xanthomonas phaseoli were characterized. On the basis of adsorption rates, latent period and burst size, plaque morphology, host range, efficiency of plating, ultrastructure, sensitivity to osmotic shock, streptomycin sensitivity, temperature effects on plating efficiency, and serology, the phages were separated into at least three groups. Some of the phages were infectio...

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Genetic transformation of streptomycin resistance in Xanthomonas phaseoli.

Transformation of resistance to antibiotics was observed in Diplococcus pneumoniae by Hotchkiss (1951, 1952a, 1952b) and in Haemophilus influenzae by Alexander and Leidy (1953) and Alexander et al. (1954). In all these species only certain strains were capable of being transformed. The ability to be transformed has been termed competence; refractory strains are not competent. Austrian (1952) ha...

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Purification and Characterization of an Endoinulinase from Xanthomonas campestris pv. phaseoli KM 24 Mutant.

An extracellular endoinulinase from Xanthomonas campestris pv. phaseoli KM 24 mutant was purified to homogeneity by gel filtration chromatography and showed a specific activity of 119 U/mg. The optimum pH and temperature of the purified enzyme were found to be 6.0 and 50 °C, respectively. The enzyme was stable up to 60 °C, retaining 60% of residual activity for 30 min, but inactivated rapidly a...

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Genetic transformation of colony type in Xanthomonas phaseoli.

The first demonstration of transformation (Griffith, 1928) was concerned with type-specific capsular polysaccharides of pneumococci. Such transformations of capsular types usually are accompanied by a change in colony type since most investigators have studied transformation of R cells which produce little or no capsular material. Coiacomitant transformation of capsular and colony type has been...

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Genetic and physiological analysis of the major OxyR-regulated katA from Xanthomonas campestris pv. phaseoli.

katA encodes the major catalase that accounts for 90 % of the total catalase activity present in Xanthomonas campestris pv. phaseoli. katA is located upstream of an ORF designated ankA encoding a cytoplasmic membrane protein homologous to eukaryotic ankyrin. Transcriptional analysis of katA and ankA identified two katA transcripts: a major monocistronic katA transcript and a minor bicistronic k...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1969

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.4.3.300-308.1969